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Next-Generation Sequencing (NGS) Services

Comprehensive genomic sequencing with state-of-the-art sequencing technologies and AI-powered data interpretation for clinical and research applications

At BioCogniz, we provide cutting-edge Next-Generation Sequencing (NGS) services that deliver unparalleled accuracy, depth, and speed for your research and clinical applications. Our state-of-the-art sequencing platforms combined with rigorous quality control ensure high-quality data generation across a wide range of genomic applications from whole genome sequencing to specialized microbial and cell-free DNA analysis.

Whether you're investigating genetic diseases, studying complex transcriptomes, exploring microbial communities, or developing precision medicine approaches, our comprehensive NGS services provide the foundation for groundbreaking discoveries.

Our NGS Sequencing Services

🧬 Whole Genome Sequencing (WGS)

Complete genomic coverage capturing all coding and non-coding regions, structural variants, and complex genomic rearrangements. Ideal for comprehensive genetic analysis, population genomics, and rare disease discovery.

  • Coverage: 30X to 60X
  • Detection of SNVs, InDels, CNVs, SVs
  • High-quality reference mapping
  • De novo genome assembly available

🧬 Whole Exome Sequencing (WES)

Cost-effective sequencing focused on protein-coding regions (exons), capturing ~98% of disease-causing mutations. Perfect for clinical diagnostics, rare variant discovery, and Mendelian disease studies.

  • Coverage: 100X to 150X
  • ~60 million base pairs covered
  • High sensitivity for coding variants
  • Custom capture panels available

🧬 Whole Transcriptome Sequencing (WTS)

Comprehensive RNA sequencing including mRNA sequencing for gene expression profiling, transcript discovery, alternative splicing analysis, and non-coding RNA characterization. Unlock functional genomic insights beyond DNA sequence.

  • mRNA, lncRNA, and microRNA detection
  • Gene expression quantification (TPM/FPKM)
  • Isoform and splice variant discovery
  • Fusion transcript detection

🦠 Microbial Genomics Sequencing

Complete bacterial, viral, and fungal genome sequencing for pathogen identification, antimicrobial resistance profiling, strain typing, and evolutionary studies.

  • De novo assembly and annotation
  • Comparative genomics
  • Plasmid and phage characterization
  • AMR gene detection

🧬 16S rRNA Sequencing

Amplicon-based sequencing of the 16S ribosomal RNA gene for taxonomic classification and diversity analysis of bacterial communities. Essential for microbiome research.

  • V3-V4 or V4 region amplification
  • Taxonomic profiling to species level
  • Alpha and beta diversity metrics
  • Metagenomic analysis available

🧬 Cell-Free DNA (cfDNA) Sequencing

Ultra-sensitive sequencing of circulating cell-free DNA from blood plasma for non-invasive prenatal testing (NIPT), liquid biopsy, cancer detection, and minimal residual disease monitoring.

  • Low input requirements (10ng cfDNA)
  • Detection of low-frequency variants
  • Fragment size profiling
  • Copy number variation analysis

Sequencing Platforms We Support

We leverage the most advanced sequencing technologies to match your project requirements:

Illumina Platform

NovaSeq 6000: Ultra-high throughput for large-scale WGS, WES, and RNA-Seq projects

NextSeq 550: Mid-throughput sequencing for targeted panels and transcriptomics

MiSeq: Small genome sequencing, amplicon sequencing, and targeted resequencing

Pacific Biosciences (PacBio)

Sequel IIe: HiFi long-read sequencing for structural variant detection, phasing, and complete genome assembly

Applications: De novo assembly, full-length transcripts, epigenetic profiling

Oxford Nanopore

PromethION: Ultra-long reads (>100kb) for complex genome resolution

MinION: Portable real-time sequencing for rapid pathogen identification

Applications: Structural variants, methylation detection, rapid diagnostics

Why Choose BioCogniz for NGS Sequencing?

🎯 99.9% Base Accuracy Industry-leading accuracy with rigorous quality control at every step
⚡ Fast Turnaround Time Results delivered in 2-4 weeks depending on sequencing depth and project complexity
📈 Flexible Coverage Options From 10X screening to 150X deep coverage, customized to your needs
Low Input Requirements Work with limited samples as low as 10ng DNA or 100ng RNA
Expert Technical Support PhD-level scientists guide you through experimental design and troubleshooting
🔐 Secure Data Management HIPAA-compliant, encrypted storage with customizable data retention policies

AI-Powered Quality Control

What sets BioCogniz apart is our integration of machine learning algorithms throughout the sequencing workflow:

Sample Requirements & Specifications

DNA Input (WGS/WES):
Minimum 100ng, optimal 1-5μg | Purity: A260/280 = 1.8-2.0 | Integrity: DIN ≥ 7.0
RNA Input (WTS):
Minimum 100ng total RNA | Purity: A260/280 = 1.8-2.1 | Integrity: RIN ≥ 7.0
cfDNA Input:
Minimum 10ng, optimal 20-50ng | Fragment size: 150-200bp
Microbial DNA:
Minimum 50ng genomic DNA | High molecular weight preferred
Sample Shipping:
DNA/RNA on dry ice | Room temperature for stabilized samples
Turnaround Time:
Standard: 2-4 weeks | Express: 7-10 days (available for select applications)

Applications Across Research & Clinical Fields

Clinical & Medical Research

Population & Evolutionary Genomics

Microbiology & Infectious Disease

Agriculture & Environmental

Deliverables

We provide comprehensive data packages tailored to your needs:

Ready to Start Your NGS Project?

Partner with BioCogniz for state-of-the-art sequencing services backed by expert support

Schedule Consultation Request Quote

Frequently Asked Questions

Q: What sequencing platforms do you use?

A: We support all major platforms including Illumina (NovaSeq, NextSeq, MiSeq), PacBio (Sequel IIe), and Oxford Nanopore (PromethION, MinION). Platform selection depends on your application, read length requirements, and throughput needs.

Q: How much DNA or RNA do I need?

A: Minimum inputs vary by application: 100ng for WGS/WES, 100ng for RNA-Seq, 10ng for cfDNA. We can work with limited samples, contact us to discuss your specific requirements.

Q: What is the turnaround time for sequencing?

A: Standard turnaround is 2-4 weeks from sample receipt, depending on sequencing depth and project size. Express services (7-10 days) are available for urgent projects.

Q: Do you provide data analysis services?

A: Yes! While this page focuses on sequencing services, we offer comprehensive bioinformatics analysis including alignment, variant calling, differential expression, and pathway analysis. Visit our NGS Data Analysis page for more information.

Q: Can you sequence samples from any organism?

A: Yes, we sequence DNA and RNA from any organism: human, animal, plant, bacterial, viral, and fungal samples. Custom reference genomes can be used for alignment and annotation.

Q: How is my data secured?

A: We use bank-grade encryption, HIPAA-compliant storage, and secure data transfer protocols. We can sign Business Associate Agreements (BAAs) for clinical projects. Data retention policies are customizable to your requirements.

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